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Cronobacter

Cronobacter is a genus of Gram-negative, facultatively anaerobic, oxidase-negative, catalase-positive, rod-shaped bacteria of the family Enterobacteriaceae. They are generally motile, reduce nitrate, use citrate, hydrolyze esculin and arginine, and are positive for L- ornithine decarboxylation. Acid is produced from D- glucose, D- sucrose, D- raffinose, D- melibiose, D- cellobiose, D- mannitol, D- mannose, L- rhamnose, L- arabinose, D- trehalose, galacturonate and D- maltose. Cronobacter spp. are also generally positive for acetoin production ( Voges–Proskauer test) and negative for the methyl red test, indicating 2,3-butanediol rather than mixed acid fermentation. The type species of the genus Cronobacter is Cronobacter sakazakii comb. nov. The genome of one strain of Cronobacter sakazakii (BAA-894) has been sequenced and annotated. According to multilocus sequence analysis (MLSA) the genus originated ~40 MYA, and the most clinically significant species, C. sakazakii, was distinguishable ~15-23 MYA. .