What is "autoradiograph"

radiograph \ra"di*o*graph\ (r[=a]"d[i^]*[-o]*gr[a^]f), n.

1. An instrument for measuring and recording solar radiation.

2. An image or picture produced upon a sensitive surface, as of a photographic or fluorescent plate, by some form of penetrating radiation other than light, as X-rays, beta rays, etc.; esp., a picture of the internal structure of opaque objects traversed by the rays; a skiagraph. When the picture is produced upon photographic film by X-rays, the picture is usually called an X-ray photo or X-ray. When an image is produced on photographic film by a radioactive substance in close proximity to the film, in a manner so as to record the spatial distribution of the radioactive substance, the resulting image is called an autoradiograph or {autoradiogram}.

n. (context photography English) A photograph image produced by placing a film in contact with a specimen containing (or treated with) radioactive material; an autoradiogram vb. (context transitive English) To subject to autoradiography.

n. a radiogram produced by radiation emitted by the specimen being photographed

An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance. Alternatively, the autoradiograph is also available as a digital image (digital autoradiography), due to the recent development of scintillation gas detectors or rare earth phosphorimaging systems. The film or emulsion is apposed to the labeled tissue section to obtain the autoradiograph (also called an autoradiogram). The auto- prefix indicates that the radioactive substance is within the sample, as distinguished from the case of historadiography or microradiography, in which the sample is X-rayed using an external source. Some autoradiographs can be examined microscopically for localization of silver grains (such as on the interiors or exteriors of cells or organelles) in which the process is termed micro-autoradiography. For example, micro-autoradiography was used to examine whether atrazine was being metabolized by the hornwort plant or by epiphytic microorganisms in the biofilm layer surrounding the plant.