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Autodisplay

Autodisplay is a genetic engineering technique which is used to insert a protein of interest on the outer surface of gram-negative bacteria. This is accomplished by attaching the protein of interest to a protein which is known to localize to the surface of the bacterial outer membrane. First introduced in the 1990s, the technique is now widely-used in research science and in biotechnology to manipulate bacteria for protein studies, drug discovery, and vaccine development.