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PKI may refer to:

  • Kings Island, an amusement park formerly known as Paramount's Kings Island
  • Partai Komunis Indonesia, the Communist Party of Indonesia
  • Peter Kiewit Institute, an Information Technology and Engineering school of the University of Nebraska system
  • Protein kinase inhibitor, a type of enzyme inhibitor that specifically blocks the action of one or more protein kinases
  • Public key infrastructure, a computer security technology