NKO can refer to:
- The N'Ko script and written language of West Africa
- People's Commissariat of Defence ( translit. Narodny Komissariat Oborony) of the Soviet Union existed in 1934 - 1946.
- Nagorno-Karabakh, common three-letter abbreviation for the country.
NKo is a Unicode block containing characters for the Manding languages of West Africa, including Bamanan, Jula, Maninka, Mandinka, and a common literary language, Kangbe, also called N'Ko.
N'Ko became part of Unicode with version 5.0 in July 2006.