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Neoschizomer

Neoschizomers are restriction enzymes that recognize the same nucleotide sequence as their prototype but cleave at a different site. In some special applications this is a very helpful feature.

For example:Prototype MaeII A^CGT produces DNA fragments with a 2-base 5' extension Neoschizomer TaiI ACGT^ produces DNA fragments with a 4-base 3' extension

Prototype ApaI GGGCC^C produces DNA fragments with a 4-base 3' extension Neoschizomer Bsp120I G^GGCCC produces DNA fragments with a 4-base 5' extension

There are also other pairs of neoschizomers.

Neoschizomers are a subset of isoschizomers.