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MLO may refer to:

  • Malibu Locals Only, a group of youths local to Malibu, California, US
  • Medio-lateral Oblique, a standard view used in mammography
  • Milos Island National Airport, Greece, IATA code
  • Misanthropic Luciferian Order, a Satanic organisation
  • Mortgage Loan Originator Housing and Economic Recovery Act of 2008 term for "loan officer"
  • Mount Laguna Observatory, near San Diego, California, US
  • Mycoplasma-like organism, earliest name for phytoplasmas