Wikipedia
Multiple Annealing and Looping Based Amplification Cycles (a.k.a. MALBAC) is a quasilinear whole genome amplification method. Unlike conventional DNA amplification methods that are non-linear or exponential (in each cycle, DNA copied can serve as template for subsequent cycles), MALBAC utilizes special primers that allow amplicons to have complementary ends and therefore to loop, preventing DNA from being copied exponentially. This results in amplification of only the original genomic DNA and therefore reduces amplification bias. MALBAC is “used to create overlapped shotgun amplicons covering most of the genome”. For next generation sequencing, MALBAC is followed by regular PCR which is used to further amplify amplicons.
Usage examples of "malbac".
I will introduce you to all my wise men and my statesmen, bah, and certainly to Casta and the Princess Hirga.
Cynthia Rhee stayed behind at the Bah Hai, and when Timothy Bell flew in to Canaveral for some hours on the simulators, she quietly moved in with him, for she needed some specific quotes on how it felt to be the only civilian amidst a group of gung-ho military pilots.
They cheered him then, but there was fear in their eyes when he sent them back to trim the sails and coax every inch of speed out of the ship in her flight towards the Bah El Mandeb.
Bah, is poileno - Jura said you scorn love, refiriendo itself to you to another tribe lanconiana.
He saw a couple of the menEstrada, Anselm, Overholserwith their bahs hugged to their chests.
He plotted his course to round the tip of the Great Horn and sail down the Gulf of Aden, through the narrows of the Bah El Mandeb and into the southern reaches of the Red Sea.
Easter season, the pagan crossed the narrows of the Bah El Mandeb with a mighty army, then drove northwards up the coast seizing all the ports and forts.
The Buh Bahs and the Ma Chos knew well that a woman's place was in a man's bed.
At the same time, over his childish countenance which was, at once so impudent and so serious, so giddy and so profound, so gay and so heart-breaking, passed all those grimaces of an old man which signify: Ah bah!