KLI may refer to:
- Klingon Language Institute − promoting the constructed Klingon language
- Konrad Lorenz Institute for Evolution and Cognition Research − international center for advanced studies in theoretical biology in Klosterneuburg, Austria.
- Korean Language Institute − providing instruction in Korean as a foreign language in Seoul.
- Kli or K'li, Hebrew for "vessel", as in Kli rishon-sheni-shlishi