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JKO may refer to:

  • J. K. Organisation
  • Jacqueline Kennedy Onassis (1929–2004), First Lady of the United States
  • Jacqueline Kennedy Onassis Reservoir in Central Park, New York City
  • Junkers, a former German aircraft manufacturer
  • Kubo language, a Trans–New Guinea language of New Guinea, spoken in the plains of the Strickland River.\