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Houstoun is a surname. Notable people with the surname include:

  • John Houstoun (1744 – 1796), American lawyer and statesman from Savannah, Georgia
  • Dr. William Houstoun (botanist) (1695–1733), British surgeon and botanist
  • William Houstoun (lawyer) (1755–1813), American lawyer, Continental Congressman for Georgia
  • Sir William Houstoun ( fl. 1830s), British army officer, Governor of Gibraltar