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GNO may refer to:

  • Girls' Night Out (disambiguation)
  • G.N.O., a song by Miley Cyrus from the album Hannah Montana 2: Meet Miley Cyrus
  • Gallium Neutrino Observatory, located at the Gran Sasso laboratory; see GALLEX
  • The GNO Multitasking Environment (GNO/ME) for the Apple IIGS computer
  • Greater New Orleans