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EOF may refer to:

  • End-of-file, the computing term for an end-of-file condition or its tangible indication
  • Empirical orthogonal functions, a statistical technique for simplifying a dataset
  • Enterprise Objects Framework, a product from Apple Computer
  • End of Fashion, an Australian band
  • Electroosmotic flow, the motion of ions in a solvent environment
  • Ethyl orthoformate, a solvent in chemistry
  • Echoes of Faydwer, an expansion for the computer game EverQuest II
  • EoF, a song editing program for the free Guitar Hero clone, Frets on Fire