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DLI is a three-letter abbreviation that may refer to:

  • Daily light integral, refers to the number of photons, received (ordinary by plants) during one day in the PAR region
  • Defense Language Institute, an agency of the United States Department of Defense
  • Department of Labor and Industry, an administrative unit common to many individual state governments in the United States
  • The Durham Light Infantry, a regiment of the British Army now amalgamated into The Light Infantry Regiment
  • Donor lymphocyte (or leukocyte) infusion, a therapy used in the field of bone marrow transplantation
  • Delay line interferometer, an Optical DPSK demodulator used for converting DPSK signal from the phase-keyed signal to an intensity-keyed signal at the receiving end in optical communication
  • Digital Library of India, Indian partner in the Million Book Project, an effort to digitize the world's books and make them freely available online
  • Department of Land Information, Government of Western Australia
  • 551 in Roman numerals.