DDK abbreviation may refer to:
- In software development, a Driver Development Kit, including Microsoft Windows Driver Development Kit
- In hull codes of United States Navy, an abolished surface combatant warship category ' Hunter-Killer Destroyer'
- In medical signs, Dysdiadochokinesia, a type of cerebellar ataxia
- In molecular biology, Cdc7/Dbf4 kinase, or Dbf4-dependent kinase, — protein kinase required for initiation of eukaryotic DNA replication.