DBU may refer to:
- Dansk Boldspil-Union, in English known as the Danish Football Association
- dBu, a decibel measurement of voltage
- Duluth Business University a private university school located in Duluth, Minnesota
- Dallas Baptist University a Christian liberal arts university located in Dallas, Texas
- dbu - ISO 639-3 code of bodum dom dogo language
- DBU, short for "Defensive Back University," an American Football-related nickname given to universities, proclaiming they produce NFL-caliber defensive backs.