CBIT may refer to:
- Continuous Built-in test
- CBIT (TV), a television rebroadcaster (channel 5) licensed to Sydney, Nova Scotia, Canada, rebroadcasting CBHT
- Chaitanya Bharathi Institute of Technology, a technical university located in Gandipet,Hyderabad, India.
- Community Broadcast Initiative Tyneside, the operator of Newcastle upon Tyne community radio station NE1fm, and previously other Restricted Service Licence broadcasts
- Cobalt-precorrin-7 (C15)-methyltransferase (decarboxylating), an enzyme