What is "ampliphox"

The ampliPHOX Colorimetric Detection Technology was created as a research tool for the analysis of low-density microarrays by providing rapid, cost effective colorimetric detection with minimal user input. The combination of a simple reagent kit and a small benchtop instrument provide a powerful new detection technology for low density (<2500 spots) microarray applications and is produced by InDevR, Inc. With an investment about ten times lower than that required for a typical microarray scanner, ampliPHOX provides equivalent analytical sensitivity to fluorescence with a simple colorimetric readout.

The key to ampliPHOX Colorimetric Detection Technology is a light-initiated chemical reaction that forms solid polymer spots on the microarray. Biotinylated targets are first captured onto the microarray, and subsequently labeled with a streptavidin-coupled initiator. When a proprietary solution is added and light from the ampliPHOX Reader illuminates the array, polymer grows selectively from only locations where the biotinylated targets were captured. The process occurs in just a few minutes, and can be seen with the unaided eye and subsequently imaged with the ampliPHOX Reader.