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AFO may refer to:

  • Absolute Force Online, a video game
  • Academia Film Olomouc
  • Advanced Force Operations
  • Aero Force One, a fan club for the rock band Aerosmith
  • Afghan Film Organization
  • Afo Dodoo (born 1973), Ghanaian footballer
  • AFO Records, the first African-American owned record label in the United States
  • Air Force One (disambiguation)
  • Alien Front Online, a video game
  • Animal Face-Off, a television show
  • Animal feeding operation, as defined by the Environmental Protection Agency
  • Anime Festival Orlando, a yearly anime convention in Orlando, Florida
  • Ankle-foot orthosis, a brace for the ankle and foot
  • Anti-Fascist Organisation, a provisional organization in Burma during World War II
  • Archiv für Orientforschung, a scholarly journal
  • Arellano-Felix Organization, a drug trafficking cartel in Mexico, also known as the Tijuana Cartel
  • Association of Field Ornithologists, an ornithological organization, publishers of Journal of Field Ornithology
  • Australian Field Ornithology, a journal of BirdLife Australia
  • Authorised Firearms Officer, a police officer in the United Kingdom who has received training and authorization to carry and use firearms
  • Eloyi language (ISO-639: afo)
  • Eloyi people, also called Afo
  • Francophone Assembly of Ontario (French: )
  • Afo, thought to be the world's oldest Clove Tree