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Affimer

Affimer molecules are small, highly stable proteins that bind their target molecules with similar specificity and affinity to that of antibodies. These engineered non-antibody binding proteins are designed to mimic the molecular recognition characteristics of monoclonal antibodies in different applications. Protein engineers have attempted to improve the experimental properties of these affinity reagents, to increase their stability, make them robust across a range of temperatures and pH, offer small sizes of the reagent, and make them easy to express at high yields in E.coli and mammalian cells.