ACMI may refer to:
- A Certain Magical Index, a Japanese light novel series
- Air Combat Maneuvering Instrumentation, a set of systems that record an aircraft's in-flight data
- American College of Medical Informatics, is a college of elected fellows who have made significant and sustained contributions to the field of medical informatics
- Australian Centre for the Moving Image, an Australian institution dedicated to the moving image in all its forms.
- Art & Creative Materials Institute, Inc., an industry trade group composed of companies that manufacture art materials.
- Aircraft, Crew, Maintenance and Insurance, a type of lease contract used in aviation, more commonly known as a Wet lease