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Wiktionary
evil laugh

interj. (non-gloss definition: Parodying an ostentatious expression of evilness in online written forums, chat rooms.) n. A stereotypical laugh associated with villains in acting, sometime represented by "muahahaha."

Wikipedia
Evil Laugh

Evil Laugh is an 1986 American comedy horror film directed and starring Dominick Brascia, and co-starring Myles O'Brien, Jerold Pearson, and Kim McKamy. While the plot focuses on a group of medical students attacked by a masked killer while repairing a building over the weekend, the film touched on a tongue-in-cheek approach, with avid Fangoria reader Barney providing the voice of reason.

Usage examples of "evil laugh".

I heard his evil laugh echoing behind the helm, mocking me, and when he came near I saw the gleam of his eyes through the slit, staring at me as a wolf stares at a cornered rabbit.

Chaotica's evil laugh was cut off with a blast that seemed to light up the comet dust around them.

The spirit-wraith mocked the words with an evil laugh and came on wildly.